Specifications

Variant Details
Catalog IDC2464-01-PE-100ULSupplier Catalog IDC2464-01-PE-100UL
Size100 µlPrice N/A
Supplier N/A Package Content Mouse Anti-Human CD126 (PE) mAb SKU:C2464-01-PE, 100ul
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsELISA, Flow Cytometry, Immunohistochemistry, ImmunoprecipitationClone No.3H1836
ConjugatedConjugatedConjugate TypePE (Phycoerythrin)
IsotypeIgGImmunogenCHO cells transfected with cDNA of the IL-6 receptor.
Purification MethodPurified by Ion Exchange chromatography.GeneCD126
FormLiquidShipping TemperatureBlue Ice
Storage & Handling4°C. Do Not FreezeStorage BufferPBS, pH 7.2.
PurityHighly Purified

Description

The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component specific to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low affinity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation and Inhibition assays. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50 Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cellls from immunized BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.

Recommended Secondary Reagents:
I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC)

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability::
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

For research use only.