The IL-6 receptor system consists of two chains, IL-6R alpha and gp130. The IL-6R alpha is the binding component speciﬁc to the IL-6, but does not transduce a signal alone. On the contrary, gp130 transmits signals not only of IL-6, but also IL-6 related cytokines, i.e. LIF, OSM, CNTF, IL-11 and CT-1. The low afﬁnity binding sites for IL-6, is composed of IL-6R alpha alone. At low concentrations, soluble IL-6R alpha acts as an agonist of IL-6 activity.
Suitable for use in Flow Cytometry, Immunohistochemistry, ELISA, Immunoprecipitation and Inhibition assays. Other applications not tested.
Flow Cytometry: 1:50 Use 10ul to label 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Spleen cellls from immunized BALB/c (Iffa Credo) mice were fused with the X63/Ag.8653 mouse myeloma cell line.
Recommended Secondary Reagents:
I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC)
Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control
Storage and Stability::
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.