MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
The MMP’s are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMP’s are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-3 can degrade proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. It can activate other MMP’s and may be involved in the repair processes of the heart after myocardial infarction.
Suitable for use in FLISA, Immunohistochemistry, and Western Blot. Other applications not tested.
Immunohistochemistry (paraffin): 1:10-1:40
Requires antigen retrieval using citrate heat treatment prior to staining of paraffin sections.
Western Blot: detects band at ~50kD in cell lysates
Optimal dilutions to be determined by the researcher.
Ag8563 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.