Specifications

Variant Details
Catalog IDM2421-15B-ML490-100ULSupplier Catalog IDM2421-15B-ML490-100UL
Size100 µlPrice N/A
Supplier N/A Package Content Mouse Anti-Human Matrix Metalloproteinase 3 (MaxLight™490) mAb SKU:M2421-15B-ML490, 100ul
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsFLISA, Immunohistochemistry, Western BlotClone No.10F164
ConjugatedConjugatedConjugate TypeMaxLight
Specific ConjugateMaxLight™490IsotypeIgG
ImmunogenOvalbumin conjugated synthetic peptide corresponding to a region within the C-terminus of human MMP-3.Purification MethodPurified by Protein G affinity chromatography.
GeneMatrix Metalloproteinase 3UniProtKBP08254
FormLiquidShipping TemperatureBlue Ice
Storage & Handling4°C. Do Not FreezeStorage BufferPBS, pH 7.2.
PurityAffinity Purified

Description

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

The MMP’s are zinc-dependent endopeptidases responsible for degrading the extracellular matrix. They are also involved in cell proliferation, migration, differentiation and apoptosis. Most MMP’s are synthesised as inactive zymogens and a propeptide region must be cleaved off before the enzyme becomes active. Their expression is increased dramatically in a variety of cancer types, where it indicates invasive disease and a poor prognosis. MMP-3 can degrade proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. It can activate other MMP’s and may be involved in the repair processes of the heart after myocardial infarction.

Applications:
Suitable for use in FLISA, Immunohistochemistry, and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:10-1:40
Requires antigen retrieval using citrate heat treatment prior to staining of paraffin sections.
Western Blot: detects band at ~50kD in cell lysates
Optimal dilutions to be determined by the researcher.

Hybridoma:
Ag8563 myeloma cells with spleen cells from Balb/c mice.

Positive Control:
Gastric carinoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

For research use only.