Specifications

Variant Details
Catalog ID168881-AF-AP-100ULSupplier Catalog ID168881-AF-AP-100UL
Size100 µlPrice N/A
Supplier N/A Package Content Mouse Anti-Human TNF-alpha (AP) mAb SKU:168881-AF-AP, 100ul
General
ClonalityMonoclonalHostMouse
ReactivityHumanCross ReactivityHuman
ApplicationsImmunofluorescence, Immunohistochemistry, Immunoprecipitation, Western BlotClone No.MAb1
ConjugatedConjugatedConjugate TypeAP (Alkaline Phosphatase)
IsotypeIgGImmunogenRecombinant human TNF-alpha
Purification MethodPurified by Protein A/G affinity chromatography.GeneTNF-alpha
UniProtKBP01375 (Human)FormLiquid
Shipping TemperatureBlue IceStorage & Handling4°C. Do Not Freeze
Storage Buffer10mM PBS. No preservative added. BSA-Free.PurityPurified

Description

This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Applications: 
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
0.5-1ug/million cells 
Immunofluorescence: 1-2ug/ml 
Immunoprecipitation: 1-2ug/500ug protein 
Western Blot: 1-2ug/ml
Immunohistochemistry: Frozen: 1-2ug/ml for 30 min at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.

Note: Applications are based on unconjugated antibody.

For research use only.