Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V7026-20UG
Size (Packaging)
2 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V7026-20UG Copy Catalog ID

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Specifications
Package Contents1x20ug
ImmunogenHuman recombinant protein was used as the immunogen for this MUC-1 antibody.
Gene ID4582
UniProtKB/Swiss-Prot P15941
ReactivityHuman
ApplicationsImmunohistochemistry, Western Blot
ClonalityMonoclonal
Clone No.MCN01-1
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionWestern blot: 1-2µg/ml,Immunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC-1 antibody to be titered up or down for optimal performance.<p>1. FFPE staining REQUIRES boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 10-20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
MUC-1 Antibody [clone MCN01-1] (V7026-100UG)
https://nsjbio.com/protocols.html
MUC-1 Antibody SKU: V7026
https://www.nsjbio.com/protocols.html
Protocol: MUC-1 Antibody
http://www.nsjbio.com/tds-pdf/muc-1-antibody-muc1-mcn01-1-v7026