Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2198IHC-7ML
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2198IHC-7ML Copy Catalog ID

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Specifications
Package Contents1x7ml
ImmunogenM1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.
Gene ID4586
UniProtKB/Swiss-Prot P98088
ReactivityHuman, Mouse, Rat
ApplicationsImmunohistochemistry
ClonalityMonoclonal
Clone No.45M1
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionThe prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC5AC antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
MUC5AC Antibody Mucin-5AC [clone 45M1] (V2198IHC-7ML)
https://nsjbio.com/protocols.html
MUC5AC Antibody Mucin-5AC SKU: V2198IHC
https://www.nsjbio.com/protocols.html
Protocol: MUC5AC Antibody Mucin-5AC
http://www.nsjbio.com/tds-pdf/muc5ac-antibody-mucin-5ac-45m1-v2198