Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V3153-20UG
Size (Packaging)
2 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V3153-20UG Copy Catalog ID

Price on Request

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Specifications
Package Contents1x20ug
ImmunogenAmino acids 138-158 from the rat protein were used as the immunogen for this Myogenin antibody.
Gene ID4656
UniProtKB/Swiss-Prot P15173
ReactivityHuman, Mouse, Rat
ApplicationsELISA, Immunohistochemistry
ClonalityMonoclonal
Clone No.F5D
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe optimal dilution of the Myogenin antibody for each application should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
Myogenin Antibody [clone F5D] (V3153-100UG)
https://nsjbio.com/protocols.html
Myogenin Antibody SKU: V9053SAF
https://www.nsjbio.com/protocols.html
Protocol: Myogenin Antibody
http://www.nsjbio.com/tds-pdf/myogenin-antibody-f5d-v3153