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Get StartedNCL Antibody SKU: V2757SAFCopy URLView Product on Manufacturer’s Website
About this Product
About This Product
About this Product
NCL Antibody SKU: V2757SAFCopy URLView Product on Manufacturer’s Website
Size (Packaging)
1 available
Supplier
2
available
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Description
Full product descriptions are available for ZAGENO customers.
Get StartedSpecifications
| Specification Name | Specification Value |
|---|---|
| Package Contents | 1x100ug |
| Immunogen | SUDHL1 cell nuclear lysate was used as the immunogen for the NCL antibody. |
| UniProtKB/Swiss-Prot | P19338 |
| Reactivity | Human |
| Applications | Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot |
| Clonality | Monoclonal |
| Clone No. | 364-5 |
| Host | Mouse |
| Conjugated | Unconjugated |
| Isotype | IgG1 Kappa |
| Dilution | Flow cytometry: 0.5-1µg/10^6 cells,Immunofluorescence: 0.5-1µg/ml,Western blot: 0.5-1µg/ml,Immunohistochemistry (FFPE): 0.25-0.5µg/ml for 30 min at RT |
| Purity | Protein G affinity chromatography |
| Purification Method | Protein G affinity chromatography |
| Additional Information | Optimal dilution of the NCL antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Shipping Temperature | 4 °C |
| Storage & Handling | 4 °C |
| Storage Buffer | PBS |
| Shelf Life | 12 months |
| Shipping Condition | Wet ice |
Resources
Protocol
3 available
NCL Antibody [clone 364-5] (V2757SAF-100UG)
https://nsjbio.com/protocols.html
https://nsjbio.com/protocols.html
NCL Antibody SKU: V2757SAF
https://www.nsjbio.com/protocols.html
https://www.nsjbio.com/protocols.html
Protocol: NCL Antibody
http://www.nsjbio.com/tds-pdf/ncl-antibody-364-5-v2757
http://www.nsjbio.com/tds-pdf/ncl-antibody-364-5-v2757