Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2212-20UG
Size (Packaging)
2 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2212-20UG Copy Catalog ID

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Specifications
Package Contents1x20ug
ImmunogenHuman recombinant Neurofilament protein was used as the immunogen. (1)
Gene ID4744
UniProtKB/Swiss-Prot P12036
ReactivityHuman, Mouse, Rat
ApplicationsFlow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
ClonalityMonoclonal
Clone No.NF421
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionWestern blot: 1-2µg/ml,Flow cytometry: 0.5-1µg/10^6 cells,Immunofluorescence: 1-2µg/ml,Immunohistochemistry (FFPE): 0.25-0.5µg/ml for 30 min at RT
PurityProtein G purified Neurofilament
Purification MethodProtein G purified Neurofilament antibody
Additional InformationThe usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.<p>1. The NF421 antibody is specific to the heavy subunit.<br>2. FFPE tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
Neurofilament Antibody [clone NF421] (V2212-100UG)
https://nsjbio.com/protocols.html
Neurofilament Antibody SKU: V2212
https://www.nsjbio.com/protocols.html
Protocol: Neurofilament Antibody
http://www.nsjbio.com/tds-pdf/neurofilament-antibody-nf421-v2212