Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2213SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2213SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenThe triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.
Gene ID4744
UniProtKB/Swiss-Prot P12036
ReactivityHuman, Mouse, Rat
ApplicationsImmunohistochemistry, Western Blot
ClonalityMonoclonal
Clone No.RT97
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionWestern blot: 1-2µg/ml,Immunohistochemistry (FFPE): 0.25-0.5µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
Neurofilament Heavy Antibody [clone RT97] (V2213SAF-100UG)
https://nsjbio.com/protocols.html
Neurofilament Heavy Antibody SKU: V2213SAF
https://www.nsjbio.com/protocols.html
Protocol: Neurofilament Heavy Antibody
http://www.nsjbio.com/tds-pdf/neurofilament-heavy-antibody-rt97-v2213