Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2764SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2764SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenNGFR from A875 melanoma cells was used as the immunogen for the NGF Receptor antibody. Its epitope is within amino acids 1-160 of the extracellular domain of NGFR/NTR.
UniProtKB/Swiss-Prot P08138
ReactivityHuman
ApplicationsImmunohistochemistry
ClonalityMonoclonal
Clone No.SPM299
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionImmunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationOptimal dilution of the NGF Receptor antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
NGF Receptor Antibody [clone SPM299] (V2764SAF-100UG)
https://nsjbio.com/protocols.html
CD271 Antibody SKU: V7134SAF
https://www.nsjbio.com/protocols.html
Protocol: NGF Receptor Antibody
http://www.nsjbio.com/tds-pdf/ngf-receptor-antibody-spm299-v2764