Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2214SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2214SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenHuman NKX2.2 recombinant protein was used as the immunogen for this antibody.
Gene ID4821
UniProtKB/Swiss-Prot O95096
ReactivityHuman, Rat
ApplicationsImmunohistochemistry
ClonalityMonoclonal
Clone No.NX2/294
HostMouse
ConjugatedUnconjugated
IsotypeIgG2b Kappa
DilutionImmunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NKX2.2 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
NKX2.2 Antibody [clone NX2/294] (V2214SAF-100UG)
https://nsjbio.com/protocols.html
NKX2.2 Antibody SKU: V2214SAF
https://www.nsjbio.com/protocols.html
Protocol: NKX2.2 Antibody
http://www.nsjbio.com/tds-pdf/nkx22-antibody-nx2294-v2214