Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2358SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2358SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenNuclei of myeloid leukemia biopsy cells were used as the immunogen.
ReactivityHuman
ApplicationsFlow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
ClonalityMonoclonal
Clone No.NM95
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionFlow cytometry: 1-2µg/10^6 cells ,Immunofluorescence: 1-2µg/ml,Immunocytochemistry: 1-2µg/ml for 30 min at RT,Immunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
Nucleoli Marker Antibody [clone NM95] (V2358SAF-100UG)
https://nsjbio.com/protocols.html
Nucleoli Marker Antibody SKU: V2358SAF
https://www.nsjbio.com/protocols.html
Protocol: Nucleoli Marker Antibody
http://www.nsjbio.com/tds-pdf/nucleoli-marker-antibody-nm95-v2358