Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2086SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2086SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenPurified GST-p27 fusion protein of human origin was used as the immunogen for this p27Kip1 antibody.
Gene ID1027
UniProtKB/Swiss-Prot P46527
ReactivityHuman, Mouse, Rat
ApplicationsFlow Cytometry, Immunofluorescence, Immunohistochemistry
ClonalityMonoclonal
Clone No.SX53G8
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionFlow cytometry: 0.5-1µg/10^6 cells,Immunofluorescence: 0.5-1µg/ml,Immunohistochemistry (FFPE): 0.25-0.5µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p27Kip1 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
p27Kip1 Antibody [clone SX53G8] (V2086SAF-100UG)
https://nsjbio.com/protocols.html
p27Kip1 Antibody SKU: V2086SAF
https://www.nsjbio.com/protocols.html
Protocol: p27Kip1 Antibody
http://www.nsjbio.com/tds-pdf/p27kip1-antibody-sx53g8-v2086