Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2282IHC-7ML
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2282IHC-7ML Copy Catalog ID

Price on Request

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Specifications
Package Contents1x7ml
ImmunogenRecombinant human wild-type p53 protein was used as the immunogen for this antibody.
Gene ID7157
UniProtKB/Swiss-Prot P04637
ReactivityHuman
ApplicationsImmunohistochemistry
ClonalityMonoclonal
Clone No.BP53-12
HostMouse
ConjugatedUnconjugated
IsotypeIgG2a Kappa
DilutionThe prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
p53 Antibody N-terminus [clone BP53-12] (V2282IHC-7ML)
https://nsjbio.com/protocols.html
p53 Antibody N-terminus SKU: V2282IHC
https://www.nsjbio.com/protocols.html
Protocol: p53 Antibody N-terminus
http://www.nsjbio.com/tds-pdf/p53-antibody-n-terminus-bp53-12-v2282