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Get Startedp53 Antibody SKU: V2284IHCCopy URLView Product on Manufacturer’s Website
About this Product
About This Product
About this Product
p53 Antibody SKU: V2284IHCCopy URLView Product on Manufacturer’s Website
Size (Packaging)
1 available
Supplier
2
available
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Description
Full product descriptions are available for ZAGENO customers.
Get StartedSpecifications
| Specification Name | Specification Value |
|---|---|
| Package Contents | 1x7ml |
| Immunogen | Recombinant human wild type p53 expressed in E. coli was used as the immunogen for this antibody. Its epitope maps within the N-terminus portion (aa 20-25) of the p53 oncoprotein. |
| Gene ID | 7157 |
| UniProtKB/Swiss-Prot | P04637 |
| Reactivity | Human |
| Applications | Immunohistochemistry |
| Clonality | Monoclonal |
| Clone No. | DO-7 |
| Host | Mouse |
| Conjugated | Unconjugated |
| Isotype | IgG2b Kappa |
| Dilution | The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Purity | Protein G affinity chromatography |
| Purification Method | Protein G affinity chromatography |
| Additional Information | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the p53 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Shipping Temperature | 4 °C |
| Storage & Handling | 4 °C |
| Storage Buffer | PBS |
| Shelf Life | 12 months |
| Shipping Condition | Wet ice |
Resources
Protocol
3 available
p53 Antibody [clone DO-7] (V2284IHC-7ML)
https://nsjbio.com/protocols.html
https://nsjbio.com/protocols.html
p53 Antibody SKU: V2284IHC
https://www.nsjbio.com/protocols.html
https://www.nsjbio.com/protocols.html
Protocol: p53 Antibody
http://www.nsjbio.com/tds-pdf/p53-antibody-do-7-v2284
http://www.nsjbio.com/tds-pdf/p53-antibody-do-7-v2284