Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2907SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2907SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenRecombinant human protein was used as the immunogen for the p53 antibody.
UniProtKB/Swiss-Prot P04637
ReactivityHuman
ApplicationsImmunohistochemistry, Western Blot
ClonalityMonoclonal
Clone No.TRP/817
HostMouse
ConjugatedUnconjugated
IsotypeIgG2b
DilutionWestern blot: 0.5-1µg/ml,Immunohistochemistry (FFPE): 0.5-1µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationOptimal dilution of the p53 antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
p53 Antibody [clone TRP/817] (V2907SAF-100UG)
https://nsjbio.com/protocols.html
p53 Antibody SKU: V2907SAF
https://www.nsjbio.com/protocols.html
Protocol: p53 Antibody
http://www.nsjbio.com/tds-pdf/p53-antibody-trp-817-v2907