Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V3490-20UG
Size (Packaging)
2 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V3490-20UG Copy Catalog ID

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Specifications
Package Contents1x20ug
ImmunogenRecombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody.
Gene ID3848 (K1); 3850 (K3); 3851 (K4); 3852 (K5); 3853 (K6A); 3856 (K8);3858 (K10); 3861 (K14); 3866 (K15); 3868 (K16); 3880 (K19)
UniProtKB/Swiss-Prot Q7Z794, Q01546
ReactivityHuman
ApplicationsFlow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
ClonalityPolyclonal
HostRabbit
ConjugatedUnconjugated
IsotypeIgG
DilutionFlow cytometry: 0.5-1µg/10^6 cells,Immunofluorescence: 1-2µg/ml,Western blot: 0.5-1µg/ml for 2 hours at RT,Immunohistochemistry (FFPE): 0.25-0.5µg/ml for 30 min at RT
PurityProtein A affinity chromatography
Purification MethodProtein A affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.<p>1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
Pan Cytokeratin Antibody, Acidic + Basic (V3490-100UG)
https://nsjbio.com/protocols.html
Pan Cytokeratin Antibody, Acidic + Basic SKU: V3490
https://www.nsjbio.com/protocols.html
Protocol: Pan Cytokeratin Antibody, Acidic + Basic
http://www.nsjbio.com/tds-pdf/pan-cytokeratin-antibody-v3490