Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2777SAF-100UG
Size (Packaging)
1 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2777SAF-100UG Copy Catalog ID

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Specifications
Package Contents1x100ug
ImmunogenRecombinant full-length human protein was used as the immunogen for the PD1 antibody.
UniProtKB/Swiss-Prot Q15116
ReactivityHuman
ApplicationsImmunohistochemistry
ClonalityMonoclonal
Clone No.SPM597
HostMouse
ConjugatedUnconjugated
IsotypeIgG1 Kappa
DilutionImmunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationOptimal dilution of the PD1 antibody should be determined by the researcher.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
PD1 Antibody [clone SPM597] (V2777SAF-100UG)
https://nsjbio.com/protocols.html
PD1 Antibody SKU: V2777
https://www.nsjbio.com/protocols.html
Protocol: PD1 Antibody
http://www.nsjbio.com/tds-pdf/pd1-antibody-spm597-v2777