This content is available to ZAGENO Customers only.
Get StartedPECAM-1 Antibody SKU: V2219Copy URLView Product on Manufacturer’s Website
About this Product
About This Product
About this Product
PECAM-1 Antibody SKU: V2219Copy URLView Product on Manufacturer’s Website
Size (Packaging)
2 available
Supplier
2
available
Products purchased from the suppliers above are governed by the Suppliers' Terms, which include applicable warranties, return policies and other terms and policies. They are available via the Supplier Terms link next to each supplier's name.
Description
Full product descriptions are available for ZAGENO customers.
Get StartedSpecifications
| Specification Name | Specification Value |
|---|---|
| Package Contents | 1x20ug |
| Immunogen | Human recombinant PECAM-1 / CD31 protein was used as the immunogen for this antibody. |
| Gene ID | 5175 (Human) |
| UniProtKB/Swiss-Prot | P16284 |
| Reactivity | Human |
| Applications | Flow Cytometry, Immunohistochemistry, Western Blot |
| Clonality | Monoclonal |
| Clone No. | C31.7 |
| Host | Mouse |
| Conjugated | Unconjugated |
| Isotype | IgG1 Kappa |
| Dilution | Flow cytometry: 1-2µg/10^6 cells,Western blot: 1-2µg/ml,Immunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT |
| Purity | Protein G affinity chromatography |
| Purification Method | Protein G affinity chromatography |
| Additional Information | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PECAM-1 antibody to be titered up or down for optimal performance.<p>1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.<br>2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
| Shipping Temperature | 4 °C |
| Storage & Handling | 4 °C |
| Storage Buffer | PBS |
| Shelf Life | 12 months |
| Shipping Condition | Wet ice |
Resources
Protocol
3 available
PECAM-1 Antibody [clone C31.7] (V2219-100UG)
https://nsjbio.com/protocols.html
https://nsjbio.com/protocols.html
PECAM-1 Antibody SKU: V2219
https://www.nsjbio.com/protocols.html
https://www.nsjbio.com/protocols.html
Protocol: PECAM-1 Antibody
http://www.nsjbio.com/tds-pdf/pecam-1-antibody-c317-v2219
http://www.nsjbio.com/tds-pdf/pecam-1-antibody-c317-v2219