Brand
NSJ Bioreagents
Manufacturer
NSJ Bioreagents
Mfr. Catalog ID
V2359-20UG
Size (Packaging)
2 available
Supplier
2 available

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Description
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Shipping Condition
Wet ice

MANUFACTURER CAT. ID V2359-20UG Copy Catalog ID

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Specifications
Package Contents1x20ug
ImmunogenPhosphotyrosine conjugated to KLH was used as the immunogen for this antibody.
ReactivityBroad
ApplicationsFlow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
ClonalityMonoclonal
Clone No.PY20
HostMouse
ConjugatedUnconjugated
IsotypeIgG2b Kappa
DilutionFlow cytometry: 0.5-1µg/10^6 cells,Immunofluorescence: 1-2µg/ml,Western blot: 0.5-1µg/ml,Immunoprecipitation: 1-2µg/500µg protein lysate,Immunohistochemistry (FFPE): 1-2µg/ml for 30 min at RT
PurityProtein G affinity chromatography
Purification MethodProtein G affinity chromatography
Additional InformationThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Phosphotyrosine antibody to be titered up or down for optimal performance.<p>1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.<br>
Shipping Temperature4 °C
Storage & Handling4 °C
Storage BufferPBS
Shelf Life12 months
Shipping ConditionWet ice
Resources
Protocol
3 available
Phosphotyrosine Antibody [clone PY20] (V2359-100UG)
https://nsjbio.com/protocols.html
Phosphotyrosine Antibody SKU: V2359
https://www.nsjbio.com/protocols.html
Protocol: Phosphotyrosine Antibody
http://www.nsjbio.com/tds-pdf/phosphotyrosine-antibody-py20-v2359