Specifications

Variant Details
Catalog ID211246-ML490-100ULSupplier Catalog ID211246-ML490-100UL
Size100 µlPrice N/A
Supplier N/A Package Content Rabbit Anti-human Histone H3, dimethyl K14 polyclonal antibody SKU:211246-ML490, 100 µl
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman, Mouse, Rat
ApplicationsImmunocytochemistry, Immunofluorescence, Western BlotConjugatedConjugated
Conjugate TypeMaxLightSpecific ConjugateMaxLight™490
IsotypeIgGImmunogenSynthetic peptide corresponding to human H3K14me2.
SpecificityRecognizes DiMetyl-Histone H3-K14.DilutionWestern Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:50Optimal dilutions to be determined by the researcher.
Purification MethodPurified by affinity chromatography.GeneHistone H3, dimethyl K14
UniProtKBQ16695Additional InformationApplications are based on unconjugated antibody.
FormLiquidShipping TemperatureBlue Ice
Storage & Handling4°C Do Not FreezePurityAffinity Purified

Description

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:50Optimal dilutions to be determined by the researcher.Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

For research use only.