Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation. Other applications not tested.Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50 – 1:200Immunoprecipitation: 1:50 – 1:200Optimal dilutions to be determined by the researcher.Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note:Applications are based on unconjugated antibody.Note: Applications are based on unconjugated antibody.
|Catalog ID||211271-FITC-100UL||Supplier Catalog ID||211271-FITC-100UL|
|Supplier||N/A||Package Content||Rabbit Anti-human Histone H3.1 polyclonal antibody SKU:211271-FITC, 100 µl|
|Reactivity||Human||Cross Reactivity||Human, Mouse, Rat|
|Applications||Immunofluorescence, Immunoprecipitation, Western Blot||Conjugated||Conjugated|
|Conjugate Type||FITC (Fluorescein isothiocyanate)||Isotype||IgG|
|Immunogen||Synthetic methylated peptide corresponding to residues surrounding R8 of human histone H3.||Specificity||Recognizes Histone H3R8me2s.|
|Dilution||Western Blot: 1:500-1:2000Immunofluorescence: 1:50 – 1:200Immunoprecipitation: 1:50 – 1:200Optimal dilutions to be determined by the researcher.||Purification Method||Purified by affinity chromatography.|
|Additional Information||Applications are based on unconjugated antibody.||Form||Liquid|
|Shipping Temperature||Blue Ice||Storage & Handling||-20°C|
For research use only.