Specifications

Variant Details
Catalog ID223301-50ULSupplier Catalog ID223301-50UL
Size50 µlPrice N/A
Supplier N/A Package Content Rabbit Anti-human HLA-DOalpha polyclonal antibody SKU:223301, 50 µl
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman, Mouse, Rat
ApplicationsImmunocytochemistry, Western BlotConjugatedUnconjugated
IsotypeIgGImmunogenSynthetic peptide corresponding to amino acids 81-130 of Human HLA-DOα.
SpecificityRecognizes endogenous levels of HLA-DOalpha.DilutionWestern Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Purification MethodPurified by immunoaffinity chromatography.GeneHLA-DOalpha
UniProtKBP06340FormLiquid
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityAffinity Purified

Description

Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigen-presenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH, preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.Storage and Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

For research use only.