Specifications

Variant Details
Catalog ID038834-PE-200ULSupplier Catalog ID038834-PE-200UL
Size200 µlPrice N/A
Supplier N/A Package Content Rabbit Anti-human NCL, ID polyclonal antibody SKU:038834-PE, 200 µl
General
ClonalityPolyclonalHostRabbit
ReactivityHumanCross ReactivityHuman
ApplicationsFLISA, Western BlotConjugatedConjugated
Conjugate TypePE (Phycoerythrin)IsotypeIgG
ImmunogenNCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human NCL.DilutionFLISA: 1:1,000 Western Blot: 1:100-500
Purification MethodPurified by Protein A affinity chromatography.GeneNCL, ID
UniProtKBP19338Protein Accession No.NP_005372.2
Additional InformationApplications are based on unconjugated antibody.FormLiquid
Shipping TemperatureBlue IceStorage & Handling4°C Do Not Freeze
PurityAffinity Purified

Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.Applications:Suitable for use in Western Blot, FLISARecommended Dilution:FLISA: 1:1,000Western Blot: 1:100-500Storage and Stability:Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Note: Applications are based on unconjugated antibody.

For research use only.