VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).Applications:Suitable for use in Western Blot, FLISARecommended Dilution:FLISA: 1:1,000Western Blot: 1:100-500Storage and Stability:Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Note: Applications are based on unconjugated antibody.
|Catalog ID||043772-PE-200UL||Supplier Catalog ID||043772-PE-200UL|
|Supplier||N/A||Package Content||Rabbit Anti-mouse VPS25, CT polyclonal antibody SKU:043772-PE, 200 µl|
|Applications||FLISA, Western Blot||Conjugated||Conjugated|
|Conjugate Type||PE (Phycoerythrin)||Isotype||IgG|
|Immunogen||VPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.||Dilution||FLISA: 1:1,000 Western Blot: 1:100-500|
|Purification Method||Purified by Protein A affinity chromatography.||Gene||VPS25, CT|
|UniProtKB||Q9BRG1||Protein Accession No.||NP_115729.1|
|Additional Information||Applications are based on unconjugated antibody.||Form||Liquid|
|Shipping Temperature||Blue Ice||Storage & Handling||4°C Do Not Freeze|
For research use only.