Specifications

Variant Details
Catalog ID043772-PE-200ULSupplier Catalog ID043772-PE-200UL
Size200 µlPrice N/A
Supplier N/A Package Content Rabbit Anti-mouse VPS25, CT polyclonal antibody SKU:043772-PE, 200 µl
General
ClonalityPolyclonalHostRabbit
ReactivityMouseCross ReactivityMouse
ApplicationsFLISA, Western BlotConjugatedConjugated
Conjugate TypePE (Phycoerythrin)IsotypeIgG
ImmunogenVPS25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-144 amino acids from the C-terminal region of human VPS25.DilutionFLISA: 1:1,000 Western Blot: 1:100-500
Purification MethodPurified by Protein A affinity chromatography.GeneVPS25, CT
UniProtKBQ9BRG1Protein Accession No.NP_115729.1
Additional InformationApplications are based on unconjugated antibody.FormLiquid
Shipping TemperatureBlue IceStorage & Handling4°C Do Not Freeze
PurityAffinity Purified

Description

VPS25, VPS36 (MIM 610903), and SNF8 (MIM 610904) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. VPS25, VPS36, and SNF8 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).Applications:Suitable for use in Western Blot, FLISARecommended Dilution:FLISA: 1:1,000Western Blot: 1:100-500Storage and Stability:Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.Note: Applications are based on unconjugated antibody.

For research use only.