Specifications

Variant Details
Catalog ID145378-100UGSupplier Catalog ID145378-100UG
Size100 µgPrice N/A
Supplier N/A Package Content Sheep Anti-human Acid Phosphatase 1 polyclonal antibody SKU:145378, 100 µg
General
ClonalityPolyclonalHostSheep
ReactivityHumanCross ReactivityHuman, Mouse, Rat
ApplicationsWestern BlotConjugatedUnconjugated
IsotypeIgGImmunogenRecombinant protein corresponding to aa2-158 from human LMW‑PTP/ACP1, expressed in E. coli (UniProt # P24666).
DilutionWestern Blot: 1ug/ml Optimal dilutions to be determined by the researcher.Purification MethodPurified by immunoaffinity chromatography.
GeneAcid Phosphatase 1FormLyophilized
Shipping TemperatureBlue IceStorage & Handling-20°C
PurityAffinity Purified

Description

LMW­PTP (Low Molecular Weight Protein Tyrosine Phosphatase; also Red cell acid phosphatase 1/ACP1, HCPTP, and HAAP/Adipocyte acid phosphatase) is a 17­18kD member of the LMWPTP family, PTP superfamily of enzymes. It is widely expressed, being found in cells diverse as B and T cells, endothelial cells and vascular smooth muscle cells. LMW­PTP dephosphorylates multiple substrates, often in a cell­dependent manner. Among the substrates for LMW­PTP include PDGR beta, EphA2 and beta­catenin. PMW­PTP is found intracellularly, generally associated with two cytoplasmic pools, one in the cytosol and the other accompaning the cytoskeleton. Human LMW­PTP is 158 amino acids (aa) in length. The catalytic region spans aa9­154, and there are two acetylation sites at Ala2 and Lys156. Phosphorylation on Tyr132 and Tyr133 occurs on cytoskeleton­associated LMW­PTP, and has the effect of increasing its phosphatase activity. There are three alternative splice variants reported for LMW­PTP. One shows a 34aa substitution for aa41­74, a second shows a deletion of aa41­74, while a third contains a 35aa substitution for aa78­158. The last two isoforms may function a negative regulators of LMW­PTP activity. Full­length LMW­PTP shares 86% aa sequence identity with mouse LMW­PTP.Applications: Suitable for use in Western Blot. Other applications not tested.Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.Storage and Stability:Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For research use only.